|Position:||Associate Head of School|
|Telephone:||+44 (0)1224 262815|
Professor Susan Duthie is Associate Head of the School of Pharmacy and Life Sciences.
Her research interests include mechanisms of action of dietary nutrients on human health, the impact of plant compounds on human health, cancer and vascular disease; and the impact of micronutrients on cancer.
Duties and Responsibilities
- Associate Head of the School of Pharmacy and Life Sciences
- Translational Research Lead for the School of Pharmacy and Life Sciences
Professor Duthie’s area of expertise is how diet affects human health and particularly how nutrition influence cell function and genomic stability. She has determined mechanistically how specific phytochemicals (including flavonoids, antioxidant vitamins and folic acid) influence DNA damage and DNA repair. Her research interests are:
- Mechanisms of action of dietary nutrients on human health and disease
- Impact of plant compounds including phytophenols, flavonoids and anthocyanins on human health; cancer and vascular disease
- Effect of micronutrients on cancer risk; differential effects of diet and supplements on malignant transformation
- Impact of B vitamins and antioxidants on cognitive function and dementia in the elderly
- Impact of natural products from underused ethnic foods on human health
- The differential impact of synthetic folic acid or natural methylated folates on malignant transformation in human colon cell in vitro
- The effect of increasing fruit and vegetable consumption on biomarkers of gut and vascular health in human volunteers
- Vitamin D and colon cancer; impact on increasing vitamin D on human colon cells in vitro
- Influence of dietary phytophenols on antioxidant capacity and genomic instability in vitro and in human subjects
- FSA UK: Programme N08: Dietary surveys and nutrients in food: informed systematic review and critical comparison of analytical methods for the quantification of blood folate status in the population. SJ Duthie, S Bird, C Mayer, H Macdonald (FSA website April 2009).
Refereed Papers (Current - 2008):
- Duthie SJ, Beattie JH, Gordon M-J, Pirie LP, Reid MD, Duncan GJ, Horgan G and McNeil CJ. (2015) B vitamin deficiency alters the expression of key proteins that regulate vascular smooth muscle cell proliferation and migration in the aorta of atherosclerotic mice. Genes & Nutrition 10 (1): 446-457.
- Drew JE, Farquharson AJ, Graham W. Horgan GW, Duthie SJ, Duthie GG (2014) Stratification of study subjects detected by gene expression profiling reveals differences in postprandial cell defence system responses. Molecular Nutrition & Food Research 58 (10); 2066-2079.
- Elliot RM, de Roos B, Duthie SJ, Boiwman FG, Rubio-Aliaga I, Crosley LK, Mayer C, Horgan G, Polley AC, Heim C, Coort SL, Evelo CT, Mulholland F, Daniel H, Mariman EC and Johnson IT (2014) Transcriptome analysis of peripheral blood mononuclear cells in human subjects following a 36 hour fast provides evidence of subtle effects on genes regulating inflammation and apoptosis as well as energy metabolism. Genes & Nutrition 9; 432-443.
- Tighe P, Duthie G, Brittenden J, Vaughan N, Mutch W, Simpson WG, Duthie S, Horgan G, Thies F. (2013) Effects of wheat and oat-based whole grain foods on serum lipoprotein size and distribution in overweight middle aged people: a randomised controlled trial. PLOS ONE 8: e70436.
- Whalley LJ, Duthie SJ, Collins AR, Starr JM, Deary IJ, Lemmon H, Duthie AC, Murray AD, Staff RT (2013) Homocysteine, antioxidant micronutrients and late onset dementia. Eur. J. Nutr., 53: 277-285.
- Thies F, Masson LF, Rudd A, Vaughan, N, Tsang C, Brittenden J, Simpson WG, Duthie S, Horgan G, Duthie G. Am. J. (2012) Effect of a tomato-rich diet on markers of cardiovascular disease risk in moderately overweight, disease-free, middle-aged adults: a randomized controlled trial. Clin. Nutr., 96: 1013-1022.
- McNeil CJ, Beattie JH, Gordon M-J, Pirie LP, and Duthie SJ. (2012) Nutritional B vitamin deficiency disrupts lipid metabolism causing accumulation of proatherogenic lipoproteins in the aorta adventitia of ApoE null mice. Molecular Nutrition and Food Research 56: 1097-1105.
- Beattie JH, Gordon M-J, Duthie SJ, McNeil CJ, Horgan GW and Kwun I-S. (2012) Suboptimal dietary zinc intake promotes vascular inflammation and atherogenesis in a mouse model of atherosclerosis. Molecular Nutrition and Food Research 56: 1122-1130.
- Bouwman FG, de Roos B, Rubio-Aliaga I, Crosley LK, Duthie SJ, Mayer C, Horgan G, Polley AC, Heim C, Coort SLM, Evelo CT, Mulholland F, Johnson IT, Elliott RM, Daniel H & Mariman ECM. (2011) 2D-electrophoresis and multiplex immunoassay proteomics analysis of different body fluids and cellular components reveal known and novel markers for prolonged fasting. BMC Medical Genomics 4: 24.
- Duthie SJ. (2011) Epigenetic modifications and human pathologies: cancer and cardiovascular disease. Proc. Nut. Soc., 70: 47-56.
- Folate and cancer: how DNA damage and DNA repair impact on colon carcinogenesis. SJ Duthie. J (2011) Inherited Metabolic Disease 34: 101-109.
- Rubio-Aliaga I, de Roos B, Duthie SJ, Crosley LK, Mayer C, Horgan G, Colquhoun IJ, Le Gall G, Huber F, Kremer W, Rychlik M, Wopereis S, van Ommen B, Schmidt G, Heim C, Bouwman FG, Mariman EC, Mulholland F, Johnson IT, Polley IC, Elliott RC, Daniel H. (2011) Metabolomics of prolonged fasting in humans reveals new catabolic markers. Metabolomics 7: 375-387.
- McNeil CJ, Beattie JH, Gordon M-J, Pirie LP & Duthie SJ. (2011) Differential effects of nutritional folic acid deficiency and moderate hyperhomocysteinemia on aortic plaque formation and DNA methylation in vascular tissue from ApoE -/- mice. Clinical Epigenetics 2: 361-368.
- Whalley LJ, Murray AD, Staff RT, Starr JM, Deary IJ, Fox HC, Lemmon H, Duthie SJ, Collins Arc, Crawford JR. Maturitas (2011) How the 1932 and 1947 mental surveys of Aberdeen schoolchildren provide a framework to explore the childhood origins of late onset disease and disability. 69: 365-372.
- Bestwick CS, Ralton, RD, Milne L, Thoo Lin PK and Duthie SJ. (2011) The influence of bisnapthalimidopropyl polyamines on DNA instability and repair in Caco-2 colon epithelial cells. Cell Biology and Toxicology 27: 455-463.
- Duthie SJ, Pirie LP, Grant G, Watson AJ, Margison GP. (2010) Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation. Cancer Prevention Res., 3: 92-100.
- Duthie SJ, Horgan G, De Roos B, Rucklidge G, Reid MD, Duncan GJ, Pirie L, Basten GP and Powers HJ. J. (2010) Blood folate status and expression of proteins involved in immune function, inflammation and coagulation: biochemical and proteomic changes in the plasma of humans in response to long-term synthetic folic acid supplementation. Proteome Res., 9: 1941-
- Tighe P, Duthie G, Vaughan N, Brittenden J, Simpson WG, Duthie S, Mutch W, Horgan G, Thies F (2010) Effect of increased consumption of whole-grain foods on blood pressure and other cardiovascular risk markers in health middle-aged people: a randomised controlled trial.. AJCN 92 (4): 733-740.
- Ralton LD, Bestwick CS, Milne L, Duthie S, Kong P. Chemico (2009) Bisnaphthalimidopropyl spermidine induces apoptosis within colon carcinoma cells. Biol. Interact., 177: 1-6.
- Beattie JH, Duthie SJ, Kwun I-S, Gordon MJ. (2009) Rapid quantification of aortic lesions in apoE-null mice. J Vasc. Res., 46: 347-352.
- Crosley L K, Duthie S, Polley A, Bouwman F, Heim C, Mulholland F, Daniel H, Horgan G, Johnson I, Mariman E, Elliott R, De Roos B. (2009) Variation in protein levels obtained from human blood cells and biofluids for platelet, peripheral blood mononuclear cell, plasma, urine and saliva proteomics. Genes and Nutrition, 4: 95-102.
- Macdonald, HM, Hardcastle, AC, Duthie, G, Duthie, S, Aucott, L, Sandison, R, Lanham New SA, Shearer M, Reid DM. (2009) Changes in vitamin biomarkers during a 2-year intervention trial involving increased fruit and vegetable consumption by free-living volunteers. Br. J. Nutr., 102: 1477-1486.
- Haggarty P, Campbell D, Duthie S, Andrews K, Hoad G, Piyathilake C, McNeill G. Brit. J. (2009) Diet, deprivation and pregnancy. Nutr., 102: 1487-1497.
- De Roos, B. Duthie, SJ, Polley A, Mulholland F, Bouman F, Carolin H, Rucklidge G, Johnson I, Mariman E, Hannelore D, Elliott R. J. (2008) Methodological recommendations and analytical variability for plasma, platelet and PBMC proteomics in human nutrition intervention studies. Proteome Res., 7: 2280-2290.
- Duthie SJ, Mavrommatis, Y, Rucklidge G, Reid, M, Duncan G, Moyer MP, Pirie LP and Bestwick CS. J. (2008) The response of human colonocytes to folate deficiency in vitro: functional and proteomic analysis. Proteome Res., 7: 3254-3266.
- J Little, M Gilmour, PA Mossey, D FitzPatrick, A Cardy, J Clayton-Smith, A Hill, SJ Duthie, AE Fryer, AM Molloy and JM Scott. (2008) Folate and clefts of the lip and palate – a UK based case-control study: Part II: Biochemical and genetic analysis. The Cleft Palate-Craniofacial Journal, 45: 420-427.
- Macdonald, HM, Black, AJ, Aucott, L, Duthie, G, Duthie, S, Sandison, R, Hardcastle, AC, S Lanham New SA, Fraser, WD, Reid DM. Am. J. (2008) Effect of potassium citrate supplementation or increased fruit and vegetable intake on bone metabolism in healthy postmenopausal women: a randomised controlled trial. Clin. Nutr., 88: 465-474.
- Haggarty P, Campbell D, Duthie S, Hoad G, Piyathilake C, McNeill G. Brit. J. (2008) Folic acid use in pregnancy and embryo selection. Obstetrics and Gynaecology., 115: 851-856.
Invited Reviews/Book Chapters/Editorials/Commentaries
- Foodomics Study of Micronutrients: The Case of Folates. Duthie SJ, In Foodomics: Advanced Mass Spectrometry in Modern Food Science and Nutrition. Ed. Cifuentes A, Wiley.
- The NuGO Proof of Principle Study package – a collaborative research effort of the European Nutrigenomics Organisation. Baccini et al., (40 authors), Genetics & Nutrition 3: 147-151.
- Berry phytochemicals, genomic stability and cancer: evidence for chemoprotection at several stages in the carcinogenic process. S. J. Duthie. Molecular Nutrition and Food Research (invited review). 51: 665-674.
- Folate, DNA stability and colorectal neoplasia. S.J. Duthie, S. Narayanan, H. Powers, G. Basten, L. Sharpe and J. Little. Proc. Nutr. Soc., 63: 571-578.
- The comet assay: protective effects of dietary antioxidants against oxidative DNA damage measured using single cell gel electrophoresis. S.J. Duthie, In Oxidative stress and ageing: advances in basic science, diagnostics and intervention. Eds., R.G. Culture and H. Rodriguez , pp309-323.
- Editorial Opinion: Folic acid-mediated inhibition of human colon cancer cell growth. S.J. Duthie, Nutrition, 17: 736-737.
- The influence of folic acid on DNA stability in human cells. SJ Duthie and S Narayanan. In Dietary anticarcinogens and antimutagens: chemical and biological aspects. Chapter 3.6 pp 143-146. Eds., IT Johnson & GR Fenwick. Royal Society of Chemistry, Cambridge.
- Cigarette smoking as an indicator of oxidative stress in relation to disease pathogenesis. G.G. Duthie, J.R. Arthur and S.J. Duthie. Chapter 33. 977-993. In Handbook of Oxidants and Antioxidants in Exercise. Ed. Sen, C.K., Packer, L. and Hanninen, O. Elsevier Science, New York.
- Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. G.G. Duthie, S.J. Duthie and J.A.M. Kyle. In Nutrition Research Reviews 13: 79-106. Ed M.I. Gurr.
- Folic acid deficiency and cancer: mechanisms of DNA instability. S.J. Duthie. British Medical Bulletin, 55: 578-592.
- The role of carotenoids in modulating DNA stability and lipid peroxidation: Importance for human health. S.J. Duthie, A.R. Collins and G.G. Duthie. Chapter 8. In Subcellular Biochemistry: Fat Soluble Vitamins, 30: 181-207. Ed. J.R. Harris. Plenum Publishing Co. London.
Dr Duthie lectures in the following areas:
- B Vitamins in Health and Disease
- Epigenetics, Diet and CancerFolate and colon Cancer
- Mechanisms of Carcinogenesis
Potential PhD Titles
Theme: Optimum health & wellbeing (diet & health; natural products; vascular health; clinical dietetics; public health policy)
Diet and colon carcinogenesis:
- Inflammation and malignant transformation in human colon carcinogenesis: impact of folic acid
Fortification of flour with synthetic folic acid was introduced in the USA and Canada in 1998 to reduce the incidence of birth defects in babies. Worryingly, information from national cancer statistics in these countries suggests that fortifying foods with synthetic folic acid may have increased colon cancer incidence. Additionally, several large-scale human trials have also reported an increase in the risk of colon tumours in subjects taking folic acid supplements. Sustained inflammation and DNA damage are linked to an increased risk of colon cancer (CC). Folic acid intake may alter the inflammatory process in the body and CC risk. In this project, we are investigating the impact that high concentrations of synthetic folic acid has on inflammation and DNA damage in human colon cells. We will study whether synthetic folic acid, at the concentrations found in human blood and colon cells after food fortification, can lead to an increase in chronic inflammation, DNA damage and markers of cancer development in human colonocytes. We will establish whether exposure to high levels of synthetic folic acid induces inflammation in human colon cells and changes the characteristics of these cells, making them more prone to becoming cancerous.
- The impact of dietary phytochemicals on low grade inflammation in human colon carcinogenesis
Chronic inflammation and genomic instability are enablers of carcinogenesis. Diet is a major environmental factor that profoundly influences human health and wellbeing. Diets rich in plant products such as cereals, fruits and vegetables protect against several human degenerative pathologies including colon cancer. This project investigates how specific phytochemicals in the human diet act to modify an immune response in inflammatory cells both in the circulation and in the human gut and subsequently how they mediate changes in gut integrity that can lead to colon cancer. We will identify the major dietary compounds present in human biological samples following intervention in human studies with key plant-based food groups (soft fruits, cruciferous vegetables and cereals) that are known to protect against human colon cancer. We will show whether, and at what levels, these phytochemicals modulate an inflammatory response in human monocytes and colon fibroblasts and the impact on markers of genomic stability and malignant transformation in human colon cells.
Natural product research:
- Terminalia ivorensis and human health: impact of TI on biomarkers of genotoxicity in human cancer cells in vitro.
Terminalia ivorensis (Ivory Coast almond) is believed to have antibacterial, inflammatory and anti-diabetic properties in people and has been used widely throughout the world in ethnic-medicine. Terminalia ivorensis is rich in phytochemicals including tannins, terpenoids and several phytophenols (such as ellagic acid and quercetin) that are known potent antioxidants and cytoprotective agents in man. In this project we are investigating the cytotoxic and genotoxic effects of Terminalia ivorensis (and its major components) in human cancer cells in culture. We are assessing the cytotoxic properties of both the plant extract and key phytochemicals isolated from the extract, at medicinally-relevant concentrations. We are using a variety of in vitro biomarkers relevant to xenobiotic metabolism, cell metabolism and carcinogenesis to assess the impact of Terminalia ivorensis on genomic stability in human cancer cells in culture.